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CONTENTS
ABSTRACTS Preface Many naturally occurring systems are endowed with a peculiar attribute called life. These systems are known as living or biological systems and are at the centre of all human cultures. The attribute life has been investigated with different perspectives and motivations for ages, but it has remained a riddle. It addresses some basic questions and fundamental problems. It is easy to perceive life and identify a living system but its scientific understanding is notoriously difficult and full of contradictions. The difficulty is linked with the inability to find an exclusive property of living systems that is law like related to all features of living systems arising from the attribute life. Crick and Watson took the first decisive step in the search of the exclusive property by discovering double helix structure of deoxyribose nucleic acid (DNA) showing specific pairing of adenine with thymine and guanine with cytosine between its two strands. They also realised that the specific pairing is indicative of a copying mechanism. It took another 13 years to decipher the genetic code employed in different steps of the copying mechanism. The copying mechanism altered the prevailing paradigm of Biology to a paradigm in which the essential ingredients of living systems are biomolecules and the working rule is the fundamental dogma of Biology. All biological properties originate at and are expressed by biomolecules. The new paradigm created a new scientific frontier of understanding and predicting the behaviour of living systems from a bio-molecular perspective. Rapid progress has been in this endeavour. The behaviour of a large number of properties of living system has become comprehensible. However, there are a small number of properties whose behaviour is incomprehensible; any attempt made to understand the behaviour encounters insurmountable problems. The incomprehensible properties bring out the inadequacies of the existing paradigm and a need to go beyond the existing paradigm. These properties are precursors of new inputs in the existing paradigm. The properties of living systems are operationally demarcated into three classes, microscopic, macroscopic, and consciousness, based on measurability and comprehensibility in the existing paradigm. The properties of microscopic class are comprehensible as well as measurable, the properties of macroscopic class are not comprehensible but measurable, and the properties of consciousness class are neither comprehensible nor measurable. The incomprehensibility of some properties arises because of the basic tenets of existing paradigm. The tenets are separate identity of biomolecules, local interaction of biomolecules, incoherent and random kinetics of chemical reactions, and information transfer via movement of biomolecules. These tenets provide only a fine-grained vision of living systems. The grain size used is of biomolecules, which is the most appropriate grain size. The choice of a finer or coarser grain size will miss some important aspects of living systems. However, the depth of vision is too small in the paradigm; it only sees the properties of microscopic class and misses the cause of correlations of distant biomolecules. It is a serious shortcoming of the existing paradigm. The effects of correlations are observable as efficiency and orders at macroscopic scales. The properties characterised by efficiency and orders at macroscopic scales belong to the macroscopic class. These are measurable properties and become comprehensible in the paradigm only after an assumption of co-operative and coordinated functioning of biomolecules that generates correlations. The assumption is usually made without any obvious reason. The assumption is attributed to some unknown peculiarity of living systems called coherence and the biomolecules involved in the cooperative functioning are called cohering. Coherence makes the cohering biomolecules behave like a macroscopic structure characterised by only a few holistic parameters. The number and behaviour of parameters depend upon the nature of the macroscopic structure that is either quantum or classical. The nature of macroscopic structure determines the nature of coherence, so that coherence is either quantum or classical. Quantum coherence is intrinsic to a system while classical coherence is a consequence of information and instruction transfers among biomolecules. Quantum coherence is associated with macroscopic quantum structures and one set of holistic parameters while classical coherence is associated with classical macroscopic structures and other set of holistic parameters. The holistic parameters are theory laden and indicate the existence of coherence but usually do not allow inferring its nature. A discriminatory property is the behaviour of correlations at small intervals. The correlation among distant molecules in a quantum structure exists at all times because of its intrinsic nature. In contrast, the correlation in a classical structure will be observable only after a time gap because of a finite speed of information transfer. Such experiments are difficult to perform and many measurements have not been made. Perhaps, because of it any serious effort has not been made to include the coherence in the biological paradigm and to study its consequences. The information about the properties of consciousness class is based on subjective and non-repeatable experiences of human objects. The nature of knowledge obtained through experiences is participatory and experiences of only a few persons have been subjected to a limited form of scientific investigation. As a result, these properties are considered immeasurable and incomprehensible. The existence of such properties in non-human living systems is matter of speculation. These properties appear counter-intuitive, illogical and non-local in the existing paradigm. A similar situation is also encountered in describing the effects of non-locality of quantum systems in a classical framework. It is reasonable to expect that similar problems in two situations arise from a common cause. Since quantum nature introduces non-locality in non-living systems, the cause of non-locality in living systems should be the quantum nature of macroscopic structures. An added bonus is that it will imply quantum nature of coherence. The phenomenon of biophoton emission addresses the basic problem of coherence and provides a means to study its consequences. The phenomenon is the incessant emission of photons by a living system mainly in the visible region and of ultra weak flux. A sensitive photo multiplier tube operating in the single photon detection mode is normally used in the detection of these photons. The signals of these photons have been observed in living systems ranging from bacteria to human tissues. The signals have two characteristic features non-exponential decay characters of signals and sensitivity of signals to all essential biological and physiological activities. Both features rule out a conventional mode of photon emission and have far reaching implications. A prefix bio is added to the photons, photon signals, and photon emission to highlight the implications. The exponential decay character is a statistical result of emission from a large number of identical but independent units. Its absence in biophoton signals implies that biophoton-emitting units are correlated in a living system and biophoton emission is a manifestation of coherence. One can learn the properties and nature of coherence by studying biophoton signals. The implications of the sensitivity are also far reaching. The biological and physiological activities affecting biophotons signals belong to the macroscopic class. Further, the sensitivity is such that a biophoton signal appears to provide a faithful reflections of biological and physiological activities of the emitting system. If it is true for all macroscopic biological activities, then a biophoton signal contains decipherable and digitalised signatures of all macroscopic biological activities. The possibility has catalysed immense experimental activity of deciphering the signatures of individual biological activities and proving the uniqueness of signatures. The success of experiment activities depends upon the capability to identify the relevant parameters of biophoton signals so that the dependence of relevant parameters on physiological and environmental factors controlling biological activities can be measured. A theoretical model also specifies the relevant parameters and its formulation depends critically on the nature of coherence. In the absence of a consensus about the nature of coherence, the relevant parameters are inferred from model calculations as well as from the observed behaviour of biophoton signals. It is hoped that relevant parameters determined from the data and models will converge. The support for the quantum nature of coherence also comes from a theoretical
conjecture providing a possible explanation of the basic facts of genetic
code namely only four bases in nucleotides, a codon consisting of three
nucleotides coding for amino acids, and only twenty amino acids. These
facts emerge in a natural way if it is conjectured that the selection
of a base pair or of an amino acid is accomplished by a quantum search.
Such selections occur in fundamental biological processes of replication,
transcription and protein synthesis. The conjecture implies that the nucleotide
making quantum searches are in quantum states because only a quantum object
can make a quantum search. It raises the question of the composite quantum
state of a bunch of nucleotides; the composite will be either decoupled
or entangled. A decoupled state retains the individual identities of nucleotides
while an entangled state obliterates the identities. The two states will
have some common and different properties. The existing paradigm explores
decoupled states of nucleotides because of its narrow vision. The paradigm
implicitly assumes only the existence of decoupled states of nucleotides
and never considers the possibility of composite entangled states. As
a result, the paradigm is unsuccessful in understanding the quantum coherence
of nucleotides. An entangled state has both local and non-local features.
The local features manifest in the properties of macroscopic class and
non-local features in the properties of consciousness class. The conjecture
is appealing and integrates the hitherto comprehensible and incomprehensible
aspects of biological systems into a broad picture. The experimental vindication
of the conjecture will come from the study of properties of photons, emitted
or absorbed in transitions of quantum states. The transitions in different
types of states occur in the manifestation of properties of different
classes. The transitions of decoupled states will probably involve thermal
photons while transitions of entangled states will involve coherent photons.
Coherent photons may have some non-local affects. The phenomenon of biophoton
emission is in accordance with the broad picture and can give credence
to the broad picture by filling its missing details through the study
of biophoton signals. Biophoton emission is thus seen as crucial evidence
of a physical basis of life, which is the theme binding, a
collage of articles of this issue. These articles cover almost all aspects
of biophoton phenomenon and give status reports of the various attempts
made for understanding the issues raised above. The attempts have differing
perspectives, follow different paths, investigate different systems, and
make different promises of potential applications. This is inevitable
in a new and evolving discipline. Perhaps, all attempts have grains of
truth and life like a quantum reality traverses all available
paths at the same time. Properties of biophotons and their theoretical implications
The word biophotons is used to denote a permanent spontaneous
photon emission from all living systems. It displays a few up to some
hundred photons /(s.cm2) within the spectral range from at least 260 to
800 nm. It is closely linked to delayed luminescence (DL) of biological
tissues which describes the long term and ultra weak reemission of photons
after exposure to light illumination. During relaxation DL turns continuously
into the steady state biophoton emission, where both, DL and biophoton
emission exhibit mode coupling over the entire spectrum and a Poissonian
photo count distribution. DL is representing excited states of the biophoton
field. The physical properties indicate that biophotons originate from
fully coherent and sometimes even squeezed states. The physical analysis
provides thermodynamic and quantum optical interpretation, in order to
understand the biological impacts of biophotons. Biological phenomena
like intracellular and intercellular communication, cell growth and differentiation,
interactions among biological systems (like Gestaltbildung
or swarming), and microbial infections can be understood in terms of biophotons.
Biophotonics, the corresponding field of applications, provide
a new powerful tool for assessing the quality of food (like freshness
and shelf life), microbial infections, environmental influences and for
substantiating medical diagnosis and therapy. Biophoton emission of a lichen species Parmelia tinctorum
The properties of biophoton signals emitted by samples of lichen species
P. tinctorum are investigated. The shape of a light induced signal is
determined from 5ms onwards using successively the bin resolution of 1,
10 and 100 ms; 1000 measurements in successive bins are made at each resolution.
The measurement of the shape is repeated at various temperatures in the
range (1-40°C) in steps of 1°C. It is found that a biophoton signal
is very sensitive to temperature and different portions of the signal
show different sensitivity. The temperature dependence of the decaying
part is even qualitatively different from that of the non-decaying part.
The signal responds to temperature changes of 0.1°C in less than 1
ms. The effect of monochromatic stimulation on the strengths of the signal
and its red, blue and green spectral components are determined in the
wavelength range (400-700) nm in steps of 10nm. The signal and its broad
spectral components have similar excitation curves. The relative strength
of spectral component appears independent of the stimulating wavelength.
The shape of the decaying portion of the signal and its red, blue and
green components is also determined. The character of decay in all four
cases is non-exponential. The measurements with various interference filters
spanning the entire visible region are made with the bin size of 20s.
These measurements are qualitative because of large fluctuations but suggest
that the spectral components of a biophoton signal are distributed in
the entire visible region. The probabilities of detecting different number
of photons in the non-decaying portion are determined by making 30000
measurements in each set with the bin size of 50, 100, 200, 300, 400,
500 and 700 ms. The probabilities determine the parameters of a squeezed
state of light- the magnitude of its displacement parameter is different
but the phase of its displacement parameter and its squeezing parameter
are same for different sizes of a bin. These measurements further indicate
that the average signal strength remains constant for 19 hr. Oscillations in ultraweak photon emission of Acetabularia
acetabulum (L.) Ultraweak photon emission of dark-incubated A. acetabulum cells were
measured with the use of a sensitive electronphotomultiplier of the Hamamatsu
550 type. The photon count series were subjected to Fourier analysis for
2-1020 sec period range. The average level of photon emission in samples
containing 50 cells was approximately 40% above background. Cell cultures
were prepared at least 24 hr before the photon emission measurements and
kept un-disturbed (established cultures). This paper reports
results of Fourier analysis of a number of samples of Acetabularia cells.
In a single population cells periodicity of light emission was not defined
directly from Fourier transformation. A large number of analyses, however,
if they are combined and compared with background data, reveal a cell-culture
specific frequency pattern. The results suggest the idea that established
cell-cultures are characterized by higher intensities of long period (minutes)
oscillations occurs, while a relative decrease was observed in the short
period (few seconds) range. The long period oscillations were not detected
in cell cultures that were prepared within 1 hr before the photon emission
measurements. It is concluded that Fourier analysis of ultraweak photon
emission, even with relatively low signals, appears to be possible. It
may serve as a non-invasive tool for monitoring the physiological state
of cells, or for studying the control of intercellular dynamics. Temperature dependence of ultraweak photon emission
in fibroblastic differentiation after irradiation with artificial sunlight
Yield of ultraweak photon emission in a cell culture model for biophotonic
measurements using fibroblastic differentiation depended on the temperature
of photonic measurement. The ultraweak photon emission of medium was significantly
higher at 37°C than at 25°C and after UVB-irradiation this difference
was even more pronounced. While with cells in the medium no temperature
dependence could be determined in unirradiated samples, after UVB-irradiation
of cells an increase of biophotonic emission was observed in postmitotic
fibroblasts. While after several UVB exposures normal cells begin to absorb
the ultraviolet light, cells from patients with the disease Xeroderma
Pigmentosum loose this capacity. In view that fibroblasts play an essential
role in skin aging, skin carcinogenesis and wound healing, the biophotonic
model using the fibroblastic differentiation system provides to be a new
and powerful non-invasive tool for the development of skin science. Biophotonic patterns of optical interactions between
fish eggs and embryos The optical (non-substantial) interactions between various biological
samples have been evident in a number of cases mainly by the effects on
their functional activity and developmental patterns. However, the mechanisms
of these interactions have remained obscure. Effect of optical interaction
has been observed on the intensity and Fourier patterns of biophoton emission
of fish embryos. We demonstrate that: (1) the short-term optical interactions
are accompanied by a gradual decrease of a total emission intensity of
the interacting batches; (2) this effect is spread laterally to that part
of a batch which does not have any direct optical contacts with its partner;
and (3) the long-term optical contacts lead to a mutual exchange of spectral
characteristics of interacting batches in which the total spectral density
values are reversed (often with an overshoot). The reversal rate depends
upon the developmental distance between the optical partners and the initial
differences of their spectral characteristics. The results are discussed
in terms of a sub-radiance and Le Chatelier principle. Solid state approach in biophoton research Main characteristics of the delayed luminescence (DL) emitted in the
seconds range from biological systems is analyzed. The correlation between
change in DL and cells organization, and similarity with some characteristics
of DL from solid state system suggest to connect DL in biological system
to decay of collective electron states formed during energy and charge
transport along the macromolecular ordered structures which form the cell.
Results of a proposed soliton model are discussed, together with some
phenomenological evidence which emphasize the possibility of using DL
measurements as an intrinsic probe in biophysical investigations. Biophoton emission of human body For the first time systematic measurements of the "ultraweak"
photon emission of the human body (biophotons) have been performed by
means of a photon detector device set up in darkness. About 200 persons
have been investigated. In a particular case one person has been examined
daily over several months. It turned out that this biophoton emission
reflects, (i) the left-right symmetry of the human body; (ii) biological
rhythms such as 14 days, 1 month, 3 months and 9 months; (iii) disease
in terms of broken symmetry between left and right side; and (iv) light
channels in the body, which regulate energy and information transfer between
different parts. The results show that besides a deeper understanding
of health, disease and body field, this method provides a new powerful
tool of non-invasive medical diagnosis in terms of basic regulatory functions
of the body. Photon counting statistics analysis of biophotons
from hands The photon counting statistics of biophotons emitted from hands is studied
with a view to test its agreement with the Poisson distribution. The moments
of observed probability up to seventh order have been evaluated. The moments
of biophoton emission from hands are in good agreement while those of
dark counts of photomultiplier tube show large deviations from the theoretical
values of Poisson distribution. The present results are consistent with
the conventional d-value analysis of the second moment of probability.
Left-right asymmetry of biophoton emission from hemiparesis
patients Left-right biophoton asymmetry from the palm and the dorsum of hands
from 7 Korean hemiparesis patients were studied. There is a strong tendency
that the left-hemiparesis patients emit more biophotons from the right
than the left hands, while the right-hemiparesis patient emits more from
the left hand. Acupuncture treatment reduces dramatically the left-right
asymmetry of biophoton emission rates. However there is no systematic
difference for the patients in the emission rates from the palm and the
dorsum of hands. Registration of spontaneous photon emission from
virus-infected cell cultures: Development of experimental system Viruses are probes by which one can gain insight into cellular
structure and function. Sir M.F. Burnet Biophoton research in blood reveals its holistic
properties Monitoring of spontaneous and luminophore amplified photon emission (PE)
from non-diluted human blood under resting conditions and artificially
induced immune reaction revealed that blood is a continuous source of
biophotons indicating that it persists in electronically excited state.
This state is pumped through generation of electron excitation produced
in reactive oxygen species (ROS) reactions. Excited state of blood and
of neutrophil suspensions (primary sources of ROS in blood) is an oscillatory
one suggesting of interaction between individual sources of electron excitation.
Excited state of blood is extremely sensitive to the tiniest fluctuations
of external photonic fields but resistant to temperature variations as
reflected in hysteresis of PE in response to temperature variations. These
data suggest that blood is a highly cooperative non-equilibrium and non-linear
system, whose components unceasingly interact in time and space. At least
in part this property is provided by the ability of blood to store energy
of electron excitation that is produced in course of its own normal metabolism.
From a practical point of view analysis of these qualities of blood may
be a basement of new approach to diagnostic procedures. Biophotons from stressed and dying organisms: Toxicological
aspects Cells and organisms exposed to detrimental and toxic substances show
different responses in photon emission dependent on amount, kind and exposure
time of toxin as well as on the organism investigated. Radical reaction-generating
substances and dehydrating, lipid dissolving and protein denaturating
toxins which do not induce direct chemiluminescence resulting from reactive
oxygen species were applied. Lethal doses of toxins and stress factors
such as osmotics and temperature evoke increase in the intensity of photon
emission resulting from a rapid and irreversible perturbation of homeostasis.
Bacterial and fungal toxins that elicit hypersensitive death of plant
cells or defense response correlated with photon emission are also briefly
discussed. Collective molecular interactions contribute to the photon-generating
degradative processes in stressed and dying organisms. The measurements
of biophoton signals and analysis of their parameters are used to elucidate
the possible mechanisms of the toxin-organism interaction and the resistance
of organisms. Toxicological perspectives of the use of these sensitive
and rapid measurements as a part of direct toxicity assessment are discussed.
Endogenous enzyme reactions closely related to photon
emission in the plant defense response Lipoxygenase (LOX) and peroxidase (POD) reactions, which are involved
in the production of reactive oxygen and radical species, are shown to
be associated with ultraweak photon emission in plant defense mechanisms.
These enzyme reactions induced high-level ultraweak photon emission in
an in vitro reaction system. The application of LOX to sweet potato slices
caused photon emission directly in plants. LOX substrate promoted photon
emission in chitosan-treated sweet potato, and LOX inhibitor markedly
suppressed this emission. Therefore, a LOX-related pathway, including
LOX and other downstream reactions, is principally associated with photon
emission in plant defense mechanisms. Variability of spectra of laser-induced fluorescence
of colonic mucosa: Its significance for fluorescence detection of colonic
neoplasia To determine the extent of a natural variability of the spectra of the
autofluorescence and its significance for a reproducibility of different
approaches typically used in studies on fluorescence detection of colonic
lesions. Two independent series of experiments have been conducted during
three years in the same laboratory. Macroscopic tissue specimens obtained
during operations of patients with colonic cancers were studied in vitro.
The tissues were excited using UV lines of c.w. He-Cd laser and pulsed
nitrogen laser and the autofluorescence spectra were recorded for areas
visually diagnosed as normal or pathologically changed mucosa. Natural
variability of the autofluorescence spectra of colonic tissues seems to
be most important factor limiting sensitivity and specificity of the diagnostic
algorithms. The mean fluorescence spectra obtained for normal mucosa and
its neoplastic lesions differ significantly but the differences are difficult
to observe because of the high natural variability among the individual
spectra. Further studies of biological basis of the colonic autofluorescence
are necessary for a progress in the field of fluorescence detection of
colonic neoplastic lesions. Time-slot modulated electromagnetic fields of wireless
communication systems: Is there a health risk for man? The safety guidelines of ICNIRP on bio-effects of low energy fields are
based absorption and transformation into thermal effects. These guidelines
are much higher than for acute reactions and long time exposure. It is
pointed out that the guidelines for cordless telephone and mobile phone
should correspond to long time exposure to low energetic electromagnetic
fields. Quantum coherence of biophotons and living systems
Coherence is a property of the description of the system in the classical
framework in which the subunits of a system act in a cooperative manner.
Coherence becomes classical if the agent causing cooperation is discernible
otherwise it is quantum coherence. Both stimulated and spontaneous biophoton
signals show properties that can be attributed to the cooperative actions
of many photon-emitting units. But the agents responsible for the cooperative
actions of units have not been discovered so far. The stimulated signal
decays with non-exponential character. It is system and situation specific
and sensitive to many physiological and environmental factors. Its measurable
holistic parameters are strength, shape, relative strengths of spectral
components, and excitation curve. The spontaneous signal is non-decaying
with the probabilities of detecting various number of photons to be neither
normal nor Poisson. The detected probabilities in a signal of Parmeliatinctorum
match with probabilities expected in a squeezed state of photons. It is
speculated that an in vivo nucleic acid molecule is an assembly of intermittent
quantum patches that emit biophoton in quantum transitions. The distributions
of quantum patches and their lifetimes determine the holistic features
of biophoton signals, so that the coherence of biophotons is merely a
manifestation of the coherence of living systems. Single photon detectors for biology : Present and
future A summary of the status of present technology for the detection of single
photons is presented with a view towards applications in biophotonics.
Included are careful discussions of the numerous problems that can be
encountered and how to get around them with the hope that this will be
of help to biologists interested in doing work in the field of biophotonics.
Emphasis is placed on traditional devices, but the field is one which
is continuously developing and we review the status of new and very interesting
technologies which are becoming available. The paper is meant to be fairly
self-contained and assumes no extensive knowledge of the physics of photodetection.
Virtual photons as carriers of consciousness
A critical comment
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