Prof. Xun Shen 1. Members of the Group Mr. Xun Shen, Professor in Biophysics, Principal investigator Miss. Zhaoxia Liu, Research Assistant Mr. Jiang Liu, Ph.D. Student Miss. Weiming Ci, Ph.D. Student Mr. Mingxin Ouyang, Ph.D. Student Mr. Yong Zhang, Ph.D. Student Mr. Huaiyong Chen, Ph.D. Student Miss. Yi Zheng, Ph.D. Student
2. Brief Curricula of the Principal Investigator
Shen Xun, Professor in Biophysics and Supervisor for Ph.D.student Shen Xun, Professor in Biophysics and Supervisor for Ph.D.student. Born in 1941 in Chongqing, China. After 6 year’s study, Graduated from Tsinghua University (Beijing, China) as a physicist in 1965. Further study on Radiation Biology in Bartholomew’s Medical College, London University, London during the period of 1973-1975. As visiting scientist, worked in the Institute for Biophysics, Giessen University, Germany in 1987.11-1989.4. Promoted to Associated professor in 1986 and became full professor in 1992 in the Institute of Biophysics, Chinese Academy of Sciences. Used to be served as a member of the Editorial Board for International Journal of Radiation Biology during the period of 1983-1990. Served as the member of Scientific Program Committee for the 11th International Congress on Radiation Research (Edinburgh, 1987) and the member of the Scientific Advisory Committee for the 11th International Congress on Photobiology (Kyoto, 1992). Now, serving as the National Committee member of China Association for Science and Technology (CAST), Vice-President and General Secretary for Biophysical Society of China, member of the Task Force for Capacity Build, International Union of Pure and Applied Biophysics (IUPAB), member of the Commission on Biological Physics, International Union of Pure and Applied Physics (IUPAP).

3. Main research subjects

3.1. Signal transduction for the stimulated respiratory burst in human polymorphonuclear leukocytes.

1. How intracellular calcium regulates the fMLP- or PMA-stimulated respiratory burst in neutrophils?
2. How cytoskeleton regulates the fMLP- stimulated respiratory burst in neutrophils.
3. The role of phosphatidylinositol 4-kinase in the fully activation of phospholipase C (PLC) and phosphatidylinositol 3-kinase (PI3K) signaling pathways.
4. Interaction between the PI3K signaling and the PLC signaling.

3.2. Signal transduction in transferring receptor-mediated cellular iron uptake

1. Kinetics of transferrin receptor-mediated iron uptake in erythroleukemic cells
2. The endocytosis, exocytosis and recycling of transferring-transferrin receptor complex.
3. The role played by reactive oxygen species (ROS) as a second messenger in transferrin receptor-mediated iron uptake.

3.3. Regulation of gene expression

1. Study on some disease-related gene expression regulated by reactive oxygen using Nox-1 gene-trasfected cells.
2. Signal transduction study in single living cell

3.4. Biophoton research

1. Photon counts statistics in living organisms.
2. Possible cell-to-cell communication through photon emission from cells.

4. Publication list

4.1. Since 1986

In international journals:

1. Shen Xun^*, Pang Suzhen, Zhang Yin, Fu Yingxian and Li Xinyuan, Cellular uptake of different components in HPD, Photobiochem. Photobiophys., 13 (1986), 165-178.

2. Shen Xun^*, Tian Jingdong, Zhu Zhaonan and Li Xinyuan, Chemiluminescence study on theperoxidationoflinoleic acid initiated by the reaction of ferrous iron with hydrogen peroxide, Biophys.Chem, 40 (1991), 161-167.

3. Shen Xun^*, Han Xingcai, Tian Jingdong, Zhao Fengyu, Xu Lingmei and Li Xinyuan, Spontaneous luminescence fromthe soybean rhizobium bacteroids, FEMS Microbiol.Lett., 81 (1991), 335-340.

4. Xun Shen^*, Jingdong Tian, Jun Li, Xinyuan Li and Yuling Chen, Formation of the excited ferryl species following Fenton reaction, Free Radical Biol.Med., 13 (1992), 585-592.

5. X.Shen^*, F.Liu and X.Y.Li, Experimental Study on the photocount statistics of the ultraweak photon emission from some living organisms, Experientia, 49 (1993), 291-295.

6. X.Shen^*, W.P.Mei and X.Xu, Activation of neutrophils by a chemically separated but optically coupled neutrophil population undergoing respiratory burst, Experientia, 50 (1994), 963-968.

7. Jian Zhang and Xun Shen*, Antioxidant Activities of Baicalin, Green Tea Polyphenols and Alizarin in vitro and in vivo, J.Nutritional Med., 7 (1997), 79-89.

8. L.X.Tang, J.L.Yang and X.Shen*, Effects of Aditional Iron-Chelators on Fe²⁺-Initiated Lipid Peroxidation: Evidence to support the Fe²⁺ ¼ Fe³⁺ Complex as the Initiator, J.Inorg.Biochem., 68 (1997),265-272.

9. Ling Bei, Tianhui Hu, Zhong Ming Qian, Xun Shen*, Extracellular Ca²⁺ Regulates the Respiratory Burst of Human Neutrophils, Biochim.Biophys.Acta, 1404(1998), 475-483.

10. Tianhui Hu, Ling Bei , Zhong-Ming Qian,Xun Shen^*, Intracellular Free Calcium Regulates the Onset of Respiratory Burst of Human Neutrophils Activated by Phorbol Myristate Acetate. Cell.Signalling, 11 (1999), 355-360

11. Tianhui Hu, Ling Bei, Zhong-Ming Qian, Xun Shen^*, W-7 primes or inhibits the fMLP-stimulated respiratory burst in human neutrophils by concentration-dependent dual expression of the formyl peptide receptor on cell surface, Biochim.Biophys.Acta, 1496 (2000), 243-251

12. Lixia Tang, Yong Zhang, Zhongming Qian and Xun Shen^*, The mechanism of Fe²⁺-initiated lipid peroxidation in liposomes: the dual function of ferrous ions, the roles of the pre-existing lipid peroxides and the lipid peroxyl radical, Biochemical J., 352 (2000), 27-36.

13. Zhong Ming Qian and Xun Shen, Brain iron transport and neurodegeneration, Trends in Molecular Medicine, 7 (2001),103-108.

14. Jiachang Yue, Jiang Liu and Xun Shen^*, Inhibition of PI4K results in a significant reduced respiratory burst in fMLP-stimulated human neutrophils, J.Biol.Chem., 276 (2001), 49093-49099.

In the books published by international publishers

15. Xun Shen^*, Wei-Ping Mei, Xing Xu, Information transfer from the neutrophils undergoing resspiratory byrst, in Biophotonics: Non-equilibrium and Coherent Systems in Biology, Biophysics and Biotechnology. (Eds. By L.V.Beloussov and F.A.Popp), Bioinform Servises Co., Moscow. 1995, pp.303315.

16. X.Shen*, W.Mei, X.Xu and F.A.Popp, Possible role of the light emission from stimulated neutrophils in their communication with others, in " Biologic Effects of Light 1995" (Eds. by Michael F.Holick and Ernst G.Jung), Walter de Gruyter, New York, 1996, pp.100-107.

17. Ling Bei,Tianhui Hu,Zhong-Ming Qian, Xun Shen*,Experimental Examination on the Possible Optical Interaction Between Two Separate Cell Populations.in Biophotons (eds.by J.J.Chang,J.Fisch,F.A.Popp), Kluwer Academic Publishers, Dordrecht/Boston/London, 1998, pp.57-64.

18. F.A.Popp, X.Shen.The Photon Count Statistic Study on the Photon Emission from Biological System Using a New Coincidence Counting System. In Biophotons (eds.by J.J.Chang, J.Fisch,F.A.Popp), Kluwer Academic Publishers, Dordrecht/Boston/London, 1998, pp.87-92.

In the journals published in China:

19. Fu Yinxian, Shen Xun, Li Qinmei and Zhang Youji, NMR study on the major componentsin hematoporphyrin derivative YHPD, Science in China, 32 (1989), 442-457.(in English)

20. Ma Yuqing, Yu Mingzhu, Son Xueling and Shen Xun, Photodynamic action of hematoporphyrin derivatives on the transformation of Bacillus subtilis, Chinese Science Bulletin, 10 (1986), 779-781. (in English)

21. Pang Suzhen, Ma Haiguan, Shen Xun, Zhang Yin, Zhou Huijuan, Fu Yinxian and Li Xinyuan, The optical absorption and fluorescence property of YHPD and its components in CHO cells, Acta Biophysica Sinica, 3 (1987), 120-125.

22. Shen Xun, Fu Shimi, Zhang Yuejing, Li Xinyuan, Dai Jun and Yu Mingzhu, Detection of ultra-weak photon emission from biological systems: low-level chemiluminescence of mungbean seedlings, rat blood and NIH 3T3 cells, Acta Biophysica Sinica, 4 (1988), 98-103.

23. Mao Dazhang, Shen Xun, Zhang Yuejing, Li Xinyuan, Dai Jun and Zhou Peizhen, Effect of metabolic inhibitor on the ultra-weak photon emission of germinating mungbean, Acta Biophysica Sinica, 4 (1988), 116-120.

24. Ma Yuqing, Yu Mingzhu, Zhang Yuejing, Li Xinyuan, Dai Jun and Shen Xun, Effect of ionizing radiation on the ultr-weak photon emission from living cells, Acta Biophysica Sinica, 4 (1988), 215-220.

25. Ma Yuqing, Yu Mingzhu, Son Xueling, Zhang Yuejing and Shen Xun Radiosensitization of mammalian cells by misonidazole and irisquinone, Acta Biophysica Sinica, 4 (1988), 331-335.

26. Ma Yuqing, Yu Mingzhu, Li Xinyuan, Zhang Yuejing, Dai Jun and Shen Xun, Effects of some chemicals on the ultra-weak photon emission from mammalian cells, Acta Biophysica Sinica, 5 (1989), 154-159.

27. Ma Yuqing, Yu Mingzhu, Zhang Yuejing, Dai Jun, Shen Xun and Wang Yingyan, Experimental study on the ultra-weak photon emission of fresh water fishes, Acta Biophysica Sinica, 5 (1989), 382-387.

28. Ma Yuqing, Yu Mingzhu, Shen Xun, Li Wuling, Zhang Wensheng, Li Renli, Radiosensitization of Chines hamster V79 cells by Nitrotriazole (NTA) derivatives, Acta Biophysica Sinica, 6 (1990), 68-71.

29. Ma Yuqing, Zhang Yuejing, Yu Mingzhu, Li Xinyuan, Dai Jun, Shen Xun, Mi Fushun, Wang Jiyi, Shen Yu and Wang Jianzhang, The ultra-weak photon emission from human blood, Acta Biophysica Sinica , 6 (1990),159-163.

30. Shen Xun, Hu Yiwei, Repair models of cell survival and corresponding computer programfor survival curve fitting, Chinese J. Radiol.Med. Protection, 11 (1991), 6:374-379.

31. Shen Xun, The dose and dose rate effectiveness factors in radiation biology, Radiation Protection, 12 (1992) 1-7.

32. Xu Wei, Shen Xun^*, Tang Lixia and Li Xinyuan, Direct observation of the ferryl radical Acta Biophysica Sinica, 10 (1994), 311-316.

33. Tang Lixia, Shen Xun, Xu Wei, Li Xinyuan, Chemiliminescence study on the lipid peroxidation initiated by ferrous ions, Acta Biophysica Sinica, 10 (1994), 297-303.

34. Xu Wei, Shen Xun^*, Tang Lixia, Zhang Jian, Study on the reaction of ferryl radical with linoleic acid, Acta Biophysica Sinica, 10 (1994), 507-512.

35. WS Zhang, X.Shen, WL Li, YF Wu, SJ Cai, XY Li, LX Tang, LJ Wang and RL Li Structure-Activity relationship for 3-nitro-1,2,4-triazoles as hypoxic tumor cell radiosensitizer, Acta Parmaceutica Sinica, 29 (1994), 739-745.

36. Shen Xun, Coherence of Biophotons, Physics, 24 (1995), 166-171.

37. Tang Lixia, Shen Xun, Methods used for lipid peroxidation assay in biological systems, Progress in Biochem. Biophys., 22 (1995); 108-111.

38. Zhang Wensheng, Wu Yanfen, Lu Wei, Li Wuling, Sun Xiaoping, Shen Xun, Li Xinyuan and Tang Lixia, Synthesis and hypoxia-selective cytotoxicity of benzofuraxans and quinoxaline di-N-oxides, Chinese J.Medicinal Chem., 5 (1995), 242-244. (in English)

39. Zhang Jian, Shen Xun^*, Tang Lixia and Li Xinyuan, Antioxidant activities of baicalin, alizarin, green tea polyphenols and trolox assayed by enhanced chemiluminescence, Acta Biophysica Sinica, 12 (1996), 348-356.

40. Li Peng, Zou Guolin and Shen Xun, Study on the chemiluminescence induced by rapid mixing bleomycin A₅ with Fe²⁺ and oxygen, Biophysica Sinica, 12 (1996), 719-723.

41. Li Peng, Zou Guolin and Shen Xun, Study on the transient fluorescence induced by the bleomycin A₅-iron complex in solution with oxygen, Biophysica Sinica, 13 (1997), 128-132.

42. Tang Li Xia, Li Xinyuan, Shen Xun, Zhang Wensheng, Wu Yanfen and Li Wuling, The radiosensitization activities of nitrotriazole derivatives and quinoxaline di-N-oxides, J.Radiat.Res.Radiat.Proces., 15 (1997), 20-24.

43. Wu Yanfen, Zhang Wensheng, Chen Xin, Hu Xiao, Li Wuling, Tang Lixia, Li Xinyuan and Shen Xun, The synthesisi of quinoxaline di-N-oxides and their hypoxia-selective cytotoxicity in vitro, Chinese J.Medicinal Chem., 7 (1997),157-161.

44. Hu Tianhui, Bei Ling and Shen Xun^*, The respiratory burst and the intra- and extracellularcalcium signal of neutrophil, Acta Biophysica Sinica, 14 (1998), 233-239.

45. Bei Ling, Hu Tianhui and Shen Xun, An improved method for isolation of neutrophils, Progress in Biochem. Biophys., 25 (1998), 4: 359-363.

46. Hu Tianhui, Bei Ling, Huang Yafei and Shen Xun^* The relationship between fMLP induced neutrophil respiratory burst and the apoptosis of neutrophil, Acta Biolgiae Experimentalis Sinica, 32 (1999), 359-366.

47. Zhang Yong, Tang Li xia and Shen Xun^*, The effect of aluminium ions on the Fe²⁺-initiated lipid peroxidation, Acta Biophysica Sinica, 15 (1999), 554-563.

48. Chuai Shaokun, Hu Tianhui, Liu Jiang and Shen Xun^* Regulation of the arachidonoc acid-stimulated respiratory burst in neutrophils by intracellular and extracellular calcium, Chinese Science Bulletin, 46 (2001), 314-317. (in English)

4.2. Before 1986

49. S.Lovell and Hsun Shen* （1976） A Flow Calorimetric Method Determining Electron Beam Energy, Phys.Med.Biol., 21, 198-208. (* Hsun is the former name of Xun).

50. Shen Xun, Pang Suzhen and Ma Haiguan (1983), A new strong characteristic optical absorption attributed to an initial photoproduct of tyrosine and phenol, Kexue Tongbao, 28, 1106-1111.

51.Shen Xun, Pang Suzhen and Ma Haiguan (1984), Flash photolysis study of insulin, Scientia Sinica B, 27,361370.

52.Tang Dejiang, Li Xinyuan, Chen Chunzhang and Shen Xun (1984), The measurement of a small transient signal superposed on a large electrical current, J.Electronics, 1,28-35.

53.Shen Xun, Pang Suzhen, Fu Yingxian and Dai Jun (1985), Determination of the reactivities of 19 common amino acids in binding radiation chemically with 14C-thymine, Kexue Tongbao, 30,829-832.

54. Ma Yuqin, Yu Mingzhu, Song Xueling and Shen Xun (1987), The photodynamic action of hematoporphyrin derivative on a transformation system of Bacillus subtilis, Kexue Tongbao, 32,1282-1285.

55. Fu Yingxian, Shen Xun, Li Qingmei and Zhang Youji (1989), NMR study on the major components in hematoporphyrin derivative YHPD, Science in China (Series B), 32,442-457.

( The above papers were published in English )

56. Shen Xun, Tang Dejiang, Chen Chunzhang, Nie Yusheng, Li Xinyuan, Zhang Yin, Guo Shengwu, Pang Suzhen, Dai Jun and Ma Haiguan (1981), A conventional flash photolysis apparatus using kinetic spectrophotometry and observation on some transient photolysis species, Acta Biochimica et Biophysica Sinica, 13,165-174.

57. Shen Xun, Pang Suzhen, He Yongke and Zhang Yin (1982), A flash photolysis study of aqueous solution of tyrosine, Acta Biochimica et Biophysica Sinica, 14,491-499.

58. Pang Suzhen, Chen Chunzhang and Shen Xun (1984), A flash photolysis study of aqueous solution of bovine serum albumin, Acta Biochimica et Biophysica Sinica, 16,533539.

59. Shen Xun, Ma Haiguan, Zhang Yin, Pang Suzhen, Fu Yingxian, Li Xinyuan, Jiang Zhonghua, Chen Xiuwei, Zhou Xiaogang, Chui Minqi and Zhang Naisen (1985), The time-resolved fluorescence studies of haematoporphyrin and relative derivatives in aqueous solution and in cells, Acta Biophysica Sinica, 1,199-205.

(above papers were published in Chinesse, but with English abstracts )

60.Shen Xun (1981), Comments on Dose-response relationship for radiation protection standards, Radiation protection, 1,72-77.

61. Chen Chunzhang, Shen Xun, Li Xinyuan, Fu Yingxian and Dai Jun (1983), A simple home-made radiochromatogram scanner and its performance, Chinese Journal of Radiological Medicine and Protection, 3(6),53-56.

62. Pang Suzhen and Shen Xun (1983), Studies on the initial photochemical process of proteins by flash photolysis method, Biochemistry and Biophysics, 1983, 3:12-17.

63. Nie Yusheng and Shen Xun (1981), The optical system in kinetic spectrophotometer, Biochemistry and Biophysics, 1981, 4: 53-58.

64. Shen Xun (1980), The fast reaction kinetics studies and radiation chemistry in biomolecules and biological systems, Guowai Yixue: Radiation Medicine, 1980, 4:3-9.

65. Shen Xun (1984), Risk assessment of ionizing radiation and its applications, Guowai Yixue: Radiation Medicine, 1984, 8:65-70.

66. Shen Xun (1984), Advances in radiotherapy, Guowai Yixue: Radiation Medicine, 1984, 8: 70-75.

67. Shen Xun (1984), The applications of synchrotron radiation in biological spectroscopy and photobiology, In: " Synchrotron Radiation and Its Applications " (Academia Sinica ed.), pp 155-161, Academia Sinica, Beijing.

68. Shen Xun (1985), Introduction on radiation biophysics, Exploration in Nature, 1985, 3:33-41

4.3. Publication on Biophoton Research

1. Shen Xun^*, Han Xingcai, Tian Jingdong, Zhao Fengyu, Xu Lingmei and Li Xinyuan, Spontaneous luminescence fromthe soybean rhizobium bacteroids, FEMS Microbiol.Lett., 81 (1991), 335-340.

2. X.Shen^*, F.Liu and X.Y.Li, Experimental Study on the photocount statistics of the ultraweak photon emission from some living organisms, Experientia, 49 (1993), 291-295.

3. X.Shen^*, W.P.Mei and X.Xu, Activation of neutrophils by a chemically separated but optically coupled neutrophil population undergoing respiratory burst, Experientia, 50 (1994), 963－968.

4. Xun Shen^*, Wei-Ping Mei, Xing Xu, Information transfer from the neutrophils undergoing resspiratory byrst, in Biophotonics: Non-equilibrium and Coherent Systems in Biology, Biophysics and Biotechnology. (Eds. By L.V.Beloussov and F.A.Popp), Bioinform Servises Co., Moscow. 1995, pp.303315.

5. X.Shen*, W.Mei, X.Xu and F.A.Popp, Possible role of the light emission from stimulated neutrophils in their communication with others, in " Biologic Effects of Light 1995" (Eds. by Michael F.Holick and Ernst G.Jung), Walter de Gruyter, New York, 1996, pp.100-107.

6. Ling Bei,Tianhui Hu,Zhong-Ming Qian, Xun Shen*,Experimental Examination on the Possible Optical Interaction Between Two Separate Cell Populations.in Biophotons (eds.by J.J.Chang,J.Fisch,F.A.Popp), Kluwer Academic Publishers, Dordrecht/Boston/London, 1998, pp.57-64.

7. F.A.Popp, X.Shen.The Photon Count Statistic Study on the Photon Emission from Biological System Using a New Coincidence Counting System. In Biophotons (eds.by J.J.Chang, J.Fisch,F.A.Popp), Kluwer Academic Publishers, Dordrecht/Boston/London, 1998, pp.87-92.

8. Shen Xun, Fu Shimi, Zhang Yuejing, Li Xinyuan, Dai Jun and Yu Mingzhu, Detection of ultra-weak photon emission from biological systems: low-level chemiluminescence of mungbean seedlings, rat blood and NIH 3T3 cells, Acta Biophysica Sinica, 4 (1988), 98-103.

9. Mao Dazhang, Shen Xun, Zhang Yuejing, Li Xinyuan, Dai Jun and Zhou Peizhen, Effect of metabolic inhibitor on the ultra-weak photon emission of germinating mungbean, Acta Biophysica Sinica, 4 (1988), 116-120.

10. Ma Yuqing, Yu Mingzhu, Zhang Yuejing, Li Xinyuan, Dai Jun and Shen Xun, Effect of ionizing radiation on the ultr-weak photon emission from living cells, Acta Biophysica Sinica, 4 (1988), 215-220.

11. Ma Yuqing, Yu Mingzhu, Li Xinyuan, Zhang Yuejing, Dai Jun and Shen Xun, Effects of some chemicals on the ultra-weak photon emission from mammalian cells, Acta Biophysica Sinica, 5 (1989), 154-159.

12. Ma Yuqing, Yu Mingzhu, Zhang Yuejing, Dai Jun, Shen Xun and Wang Yingyan, Experimental study on the ultra-weak photon emission of fresh water fishes, Acta Biophysica Sinica, 5 (1989), 382-387.

13. Ma Yuqing, Zhang Yuejing, Yu Mingzhu, Li Xinyuan, Dai Jun, Shen Xun, Mi Fushun, Wang Jiyi, Shen Yu and Wang Jianzhang, The ultra-weak photon emission from human blood, Acta Biophysica Sinica , 6 (1990),159-163.

14. Shen Xun, Coherence of Biophotons, Physics, 24 (1995), 166-171.

5. Internet Publications:

• Application of chemiluminescence and fluorescence probes in the studying cell signaling, Xun Shen, IIB-conference 2000
• The possible role played by the biophotons in the long-range interaction between neutrophil leukocytes, X. Shen, L. Bei, T.-H. Hu, B. Aryal, IIB-conference 1999