Conference on Biophotons 1999 - STRUCTURE-FUNCTIONAL RELATIONS OF BIOLOGICAL SYSTEMS CAN BE REVEALED BY STATISTICS OF SCATTERED COHERENT RADIATION

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Conference on Biophotons 1999
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STRUCTURE-FUNCTIONAL RELATIONS OF BIOLOGICAL SYSTEMS CAN BE REVEALED BY STATISTICS OF SCATTERED COHERENT RADIATION
O.N. Budagovskaya and A.V. Budagovsky

Department of Biophysics I.V.Michurin's All-Russian Research Institute for genetics and selection of fruit plants 393740 Tambov Region, Michurinsk

A coherent field can enhance functional activity of cells by changing their metabolism. A non-absorbed (scattered) part of radiation also undergoes transformations, as depending upon the microstuctural organization of an irradiated sample. It would be of interest to study to what extent biological processes can affect the properties of the interacting coherent field. We have investigated the scattering of a laser radiation by a plant tissue with its functional activity being modulated by biotical, chemical or physical agents. We have studied leaf plates and fruits of different species and stocks of cultural plants. The scattered coherent radiation have been analyzed by means of a special device permitting to evaluate quantitatively the amplitude-phasic properties of a field (Budagovskaya, Budagovsky, 1990). For evaluating a functional state of a tissue we have used several biochemical and physiological indicators, in particular those indicating the changes of a specific photosynthetic activity by a technique of PAM chlorophyll fluorescence. We have observed a considerable effect of destabilizing factors upon scattering of a coherent radiation. For example, treatment by a herbicide (paracvat), fungicide (previcur), retardant (ceron) resulted in a considerable and significant (1,5 - 3 times) decrease of a coherency degree of a testing light flow. Similar results have been obtained while using - or UV-radiation. A development of a pathogenic effect upon spinach leafs (by a pathogen, Perenespora spinaceae) led to an almost 2-fold decrease of a coherency degree whereas the photosynthetic activity of the same samples decreased no more than to 20-40%. As a total, a significant correlation (R2 > 0,7) has been determined between the phasic properties of a scattered radiation and a number of physiological and biochemical indicators. By employing a mathematical model of a phasic screen we were able to reveal analytically a relation between the micromorphological parameters of a plant tissue and the degree of coherency of a scattered optical radiation. We have tested experimentally that the changes in the field's statistics are related to the structural alteration within the functioning organism. It should be a matter of further investigations, whether this event is used for biocommunication.

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