INTERNATIONAL INSTITUTE
OF BIOPHYSICS
Conference
on Biophotons 1999
Abstracts of the Conference Reports in
alphabetic Order
BIOELECTRIC POTENTIAL OF CELL NUCLEI AS A SOURCE OF A CELLULAR
BIOLOGICAL FIELD
V.G. Shachbazov
Department of Cytology and Genetics, University of Harkov, Ukraine
We have studied bioelectrical properties of cell nuclei by means of
microelectrode technique and intercellular microelectrophoresis, with the
use of original devices and techniques. On plants' and Drosophila melanogaster
cells we could reveal the differences in the bioelectric properties of
cell nuclei, as depending upon genotype, age and external influences. We
detected also the rhythmical oscillations of bioelectric potential during
microelectrode contact with the external nuclear membrane. The deviation
of the electrokinetic potential from the initial level under the influence
of increased temperature, radiation and other damaging factors can be considered
as an indication of a cellular homeostasis, namely, a non-specific stability
of a given object.
A study of the humans' epithelial cells permitted to construct a graph
of the age-dependency of electrokinetic potential, as a well as the alterations
of this potential due to tiredness, drugs employment, intoxication and
different diseases. The new techniques and devices are now largely used
in general, recreational, sportif and labour medicine.
We were also able to demonstrate the connections between the nuclear
electrokinetic potential on one hand, and the activity of genes, state
of the chromatin, the dimensions and the shape of the cells. These cells'
properties could be modified by a laser radiation and electromagnetic waves
of a millimeters' range.
These experimental data permit to make some theoretical conclusions.
The electrical potential of a cell nuclei determining the energetical parameters
of a nuclear-cytoplasmic transfer of substances can be considered as a
realization of E. Bauer's principle of a stable non-equilibricity of the
biological systems and of Onzager's principle of the energetical gradient
driving thermodynamical forces and fluxes. Our results confirm and specify
A. Gurwitsch' concept of the cellular biological fields and the role of
a nucleus as a field source.
© International
Institute of Biophysics 2001 E-mail: iib@lifescientists.de
Previous
