INTERNATIONAL INSTITUTE
OF BIOPHYSICS
Conference
on Biophotons 1999
Abstracts of the Conference Reports in
alphabetic Order
THE COOPERATIVE EFFECT OF SEROTONIN AND WEAK MAGNETIC FIELDS
TUNED TO PARAMETRIC RESONANCE FOR IONS ON THE MORPHOGENESIS OF REGENERATING
PLANARIANS
DUGESIA TIGRINA
Z.E. Rogdestvenskaya, Kh.P. Tiras*, L.K. Srebnitskaya,
and V.V. Lednev
Institute of Theoretical and Experimental Biophysics, Institute of
Cell Biophysics* RAS Pushchino, Moscow region, 142292, Russia.
E-mail: rojdestvensk@venus.iteb.serpukhov.su
Objectives. Regenerating planarians are used as a model test-system
to study the influence of various physical and chemical factors. Amputation
of a part of the body is accompanied by the release of serotonin from the
damaged nerves, which initiates the chain of events leading to the complete
re-growth of the planarian's body. We have shown earlier the possibility
of stimulating as well as inhibiting proliferation of neoblasts by weak
(compared to that of Earth's) combined magnetic fields (CMF) [1]. The goal
of this work was to study the combined action of exogenous serotonin and
CMF tuned to parametric resonance for calcium (Ca2+-CMF) and
potassium ions (K+-CMF) according to the theory of the magnetic
parametric resonance in biosystem [2].
Methods. The quantitative measurements of blastema re-growth
by 3-th day of regeneration was performed using the in vivo computer morphometry.
It is based on recording in vivo the photocontrast between old (pigmented)
and new (transparent) parts of planarian's body. The system of on-line
computer image analysis with special software was used to calculate the
ratio of blastema area to whole body area as a quantitative parameter of
regeneration as average (n=30) for the following groups 1) without any
influence, 2) exposed to the (Ca2+-CMF) or (K+-CMF),
3) with serotonin added immediately after sectioning, 4) with simultaneous
application of both factors.
Results. It has been found that 3 day exposure of regenerating
planarians to the Ca2+-CMF accelerated the re-growth of blastema
by 305 %. Almost the same increase by 306 % have been obtained by addition
of 10-6 M serotonin immediately after sectioning without application
of the Ca2+-CMF. The activation of the re-growth of blastema
by 455 % was detected after application of both factors. The exposure of
planaria to K+-CMF suppressed blastema re-growth by 255 %. The
inhibitory influence of K+-CMF was abolished in the presence
of 10-6 M serotonin.
The data presented here and also obtained earlier clearly indicate the
possibility of regulating the processes of regeneration with the use of
weak magnetic fields. Non-additive character of the joined action of CMF
and serotonin allows us to propose the existence of the same routs in the
mechanism by which these two factors affect the morphogenesis.
References:
-
Lednev V.V., Srebnitskaya L.K., Ilyasova E.N., Rogdestvenskaya Z.E., Klimov
A.A., Belova N.A., Tiras Kh.P. (1996) Biophysics, 41(4): 815-825 (in Russian).
-
Lednev V.V. (1996) Biophysics, 41(1):224-236 (in Russian).
© International
Institute of Biophysics 2001 E-mail: iib@lifescientists.de
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