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FREQUENCY PATTERNS OF ULTRAWEAK PHOTON EMISSION (UPE) FROM THE DEVELOPING EGGS AND EMBRYOS OF A FISH, MISGURNUS FOSSILUS L.
L.V. Beloussov, A.B. Burlakov, A.A. Konradov
Faculty of Biology, Moscow State University and Institute of Biochemical
Physics, Russian Academy of Sciences.
E-mail: lbelous@soil.msu.su
One of the main difficulties in interpreting numerous biological effects of ultra-weak photon emission (UPE) produced by the living specimen is associated with its extremely low average intensity, which do not often significantly exceeds the level of a background count and is sometimes even lower. This makes but natural to suggest that the bio-effects of UPE are at least partly caused by some specificity of its frequency spectrum. We explored this hypothesis using the developing samples of a fish, M. fossilus, from freshly fertilized eggs up to freely swimming larvae. Only during early cleavage stages (2 - 16 blastomeres) directly visible UPE periodicity could be traced: each next round of cleavage divisions (coinciding with the period of DNA synthesis for the next division) is associated with the cascades of brief (0,01 s) UPE outbursts 20-40-fold exceeding the preceding UPE level.
What can be detected in any developmental stage, are more or less specific UPE frequency (Fourier) spectra which we have characterized by the following indexes:
1.Spectral densities (SD) measured in 10-1 - 100 s-1 range; 2. Cross-correlation between SDs monitored within successive overlapped time periods; 3. Second order SDs (2nd order FP)
As a result, we could demonstrate that the developing samples can be distinguished from each other and from non-living photon-emitters by: (a) more pronounced SD-peaks; (b) prolonged plateau of high correlation of Fourier spectra separated by narrow troughs of decreased correlation; similar patterns for the control samples are more smooth; (c) specific 2nd order FP, mostly pronounced after illumination pulses. By plotting 2nd order FP in log-log coordinates we could see that in the living samples (and especially in those belonging to blastula-epiboly stages) the 2nd order FP extremums (i.e. highest and lowest intensities) can be connected with each other by a much more extensive, ramified and interlaced rectilinear network (often concentrated around few dominating centers) than in the non-living samples or non-fertilized eggs. Such a network becomes even more pronounced after illumination pulses and after the optical interaction of two different batches. We could trace a progressive development of such networks during the optical contact between the two batches. We consider these network patterns as the manifestations of the interactions between different UPE oscillators both within a single batch and between the different batches which correlate their SDs to their frequencies according to a law described by power functions. The latter may indicate that each one of the 2nd order FP extremum is multiplicative rather than additive, i.e. depends upon the interactions within the entire collective of the oscillators.
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