INTERNATIONAL INSTITUTE OF BIOPHYSICS
Conference on Biophotons 1999
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BIOLUMINESCENCE AND BIOCUMMUNICATION OF Lampyridae

Jiin-Ju Chang *# and Fritz-Albert Popp#

* Institute of Biophysics, Chinese Academy of Sciences. 100101, Beijing China Fax. 0086 10 6488 8428, E-Mail. changjj@sun5.ibp.ac.cn; # International Institute of Biophysics e.V., IIB. D-41472 Neuss, Germany Fax 0049 02182/825132,
E-Mail: Biophotonik.KL@t-online.de

It is well known that male Lampyridae, commonly known as fireflies, search for females at night by emitting flashes of light in a characteristic pattern. Females respond to the patterns by flashing back at species-specific time intervals after the male flashes. This is a typical example of biocommunication by light. However there is no clear picture of how the light communication carried out. By using Chinese Lampyridae captured in Beijing Hunting park, photon emission in different conditions of the flies were measured by the double chamber with coincidence counting system (CCS) and by PMS-1 with light illuminating in Dr. Popp's Lab.in order to answer the above questions. The results show that the females respond to the male flashing only when she is in free flying conditions as showed in Fig. 1(the left). However when they were put separately in a small cuvette where they can not fly freely they will communicate in another way flashing back.

The female flies also respond light illumination but in a very special manner showed as Fig.2.(the right). The arrow shows the time of illumination with white light. After a short decrease in the intensity, the photon emission from the fly appear in an oscillation pattern with decreasing frequency.
 

Analysis of photocount statistics and cross-correlation of photon emission from the Lampyridae showed that they followed Possonian distribution and they are correlated each other.
 

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