INTERNATIONAL INSTITUTE
OF BIOPHYSICS
Conference
on Biophotons 1999
Abstracts of the Conference Reports in
alphabetic Order
MITOGENETIC EFFECT OF MEALWORM BEETLES ON SPRUCE SEEDS
GERMINATION
Igor Jerman, Romana Ruzic, Maja Berden
BION Institute for Bioelectromagnetics and New Biology, Celovška 264,
1000 Ljubljana, Slovenija, fax: +386 61 574960
1.Introduction The pioneer of the concept of a the mitogenetic effect
involving coherent high frequency electromagnetic field was A. G. Gurwitsch,
who, after many experiments with onions, came to a conclusion that the
mediator of the so called mitogenetic effect influence was the UV radiation.
Today this approach covers a well developed research field of ultraweak
biophoton emission (1). In our previous experiments we tried to see if
there exists any non-chemical influences of chosen organisms on other ones
via imprinting into water (2, 3). The results encouraged us for the present
set of experiments, where the research of direct non-chemical radiatory
influence (via a quartz test tube barrier) of mealworm beetles (Tenebrio
molitor) on germination of spruce seeds (Picea abies) has been
tried. 2.Material and methods 4 x 80 spruce seeds were grown on the moist
filter paper in a 4 Petri dishes and placed down in a 4 round plastic containers.
The same number of seeds and dishes corresponded for represented the control
groups. The seeds were previously watered for two days to simulate induce
water stress conditions. Each container was enveloped with the aluminum
foil from inside, in the middle the quartz test tube was fixed. (for details
see Figure 1). Every experimental test tube contained 5 mealworm larvae.
A control group contained no larvae. The Every experiment lasted 7 days.
The seedlings were watered with distilled water on day 1 (6 ml) and again
on day 5 (4 ml). The larvae were exchanged with fresh ones on day 4. The
whole system was kept in the dark. The illumination by artificial light
was allowed only during measurements of germination. The number of germinated
seeds was examined every day; at the end of the experiment (day 7) the
lengths of all germinated seedlings were measured and appropriately statistically
evaluated. 3.Results The results showed that the growth of seedlings was
enhanced for around 19%, when they were exposed to mealworm beetles in
spite of the fact that there was no chemical contact between them. The
effect was statistically significant at the level of 0.01 when the data
of 5 repeated experiments were pooled. The germination was mostly enhanced
in the begining of germination. 4.References: 1) Popp F A (1992): Some
essential questions of biophoton research and probable answers, in: Recent
advances in biophoton research and its applications (eds.: Popp FA, Li
KH, Gu Q), World Scientific, Singapore, 1-46. 2) Jerman I, Berden M, Ruzic
R (1996): Biological influence of ultraweak supposedly EM radiation from
organisms mediated through water. Electro- and Magnetobiology 15(3): 229-244.
© International
Institute of Biophysics 2001 E-mail: iib@lifescientists.de