INTERNATIONAL INSTITUTE OF BIOPHYSICS
Conference on Biophotons 1999
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MITOGENETIC EFFECT OF MEALWORM BEETLES ON  SPRUCE SEEDS GERMINATION
Igor Jerman, Romana Ruzic, Maja Berden


BION Institute for Bioelectromagnetics and New Biology, Celovška 264, 1000 Ljubljana, Slovenija, fax: +386 61 574960

1.Introduction The pioneer of the concept of a the mitogenetic effect involving coherent high frequency electromagnetic field was A. G. Gurwitsch, who, after many experiments with onions, came to a conclusion that the mediator of the so called mitogenetic effect influence was the UV radiation. Today this approach covers a well developed research field of ultraweak biophoton emission (1). In our previous experiments we tried to see if there exists any non-chemical influences of chosen organisms on other ones via imprinting into water (2, 3). The results encouraged us for the present set of experiments, where the research of direct non-chemical radiatory influence (via a quartz test tube barrier) of mealworm beetles (Tenebrio molitor) on germination of spruce seeds (Picea abies) has been tried. 2.Material and methods 4 x 80 spruce seeds were grown on the moist filter paper in a 4 Petri dishes and placed down in a 4 round plastic containers. The same number of seeds and dishes corresponded for represented the control groups. The seeds were previously watered for two days to simulate induce water stress conditions. Each container was enveloped with the aluminum foil from inside, in the middle the quartz test tube was fixed. (for details see Figure 1). Every experimental test tube contained 5 mealworm larvae. A control group contained no larvae. The Every experiment lasted 7 days. The seedlings were watered with distilled water on day 1 (6 ml) and again on day 5 (4 ml). The larvae were exchanged with fresh ones on day 4. The whole system was kept in the dark. The illumination by artificial light was allowed only during measurements of germination. The number of germinated seeds was examined every day; at the end of the experiment (day 7) the lengths of all germinated seedlings were measured and appropriately statistically evaluated. 3.Results The results showed that the growth of seedlings was enhanced for around 19%, when they were exposed to mealworm beetles in spite of the fact that there was no chemical contact between them. The effect was statistically significant at the level of 0.01 when the data of 5 repeated experiments were pooled. The germination was mostly enhanced in the begining of germination. 4.References: 1) Popp F A (1992): Some essential questions of biophoton research and probable answers, in: Recent advances in biophoton research and its applications (eds.: Popp FA, Li KH, Gu Q), World Scientific, Singapore, 1-46. 2) Jerman I, Berden M, Ruzic R (1996): Biological influence of ultraweak supposedly EM radiation from organisms mediated through water. Electro- and Magnetobiology 15(3): 229-244.
 

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